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mouse ifn γ elispot kit  (Cellular Technology Ltd)


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    Cellular Technology Ltd mouse ifn γ elispot kit
    Mouse Ifn γ Elispot Kit, supplied by Cellular Technology Ltd, used in various techniques. Bioz Stars score: 96/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/color+elispot+kit/bio_rxiv__64898__2026__03__11__711122-206-15-14?v=Cellular+Technology+Ltd
    Average 96 stars, based on 66 article reviews
    mouse ifn γ elispot kit - by Bioz Stars, 2026-07
    96/100 stars

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    SNAP-AD 5 immunization elicited antigen-specific B cell response without high antigen-specific T cell response in mice. (A) Representative ELISPOT wells showing IFN-γ–secreting splenocytes from SNAP-AD 5 immunized, individual antigen immunized, or untreated control mice following stimulation with medium, individual antigens, or a 5-plex antigen mix. (B) Quantification of IFN-γ spots from (A) were counted and analyzed by a two-way ANOVA followed by Bonferroni's multiple comparison test, comparing each experimental group to the control, ∗p < 0.05. (C) Representative ELISPOT wells showed B-cell producing IgG in blood from mice (n = 5) immunized with SNAP-AD 5 or untreated mice. Blood cells after lysis were stimulated with medium alone, 10 μg/mL of Igκ/Igλ antibodies, or 10 μg/mL 5-plex mixed antigen. (D) Quantification of B-cell producing IgG spots from (C) were analyzed by a one-way ANOVA with multiple comparison followed by Tukey's test, ∗ P<0.05, ∗∗∗∗P<0.0001 .

    Journal: Brain, Behavior, & Immunity - Health

    Article Title: Therapeutic nanoliposome vaccine targeting multiple Aβ and tau epitopes reduces AD-like brain pathologies and rescues cognitive deficits in 3xTg-AD mice

    doi: 10.1016/j.bbih.2025.101167

    Figure Lengend Snippet: SNAP-AD 5 immunization elicited antigen-specific B cell response without high antigen-specific T cell response in mice. (A) Representative ELISPOT wells showing IFN-γ–secreting splenocytes from SNAP-AD 5 immunized, individual antigen immunized, or untreated control mice following stimulation with medium, individual antigens, or a 5-plex antigen mix. (B) Quantification of IFN-γ spots from (A) were counted and analyzed by a two-way ANOVA followed by Bonferroni's multiple comparison test, comparing each experimental group to the control, ∗p < 0.05. (C) Representative ELISPOT wells showed B-cell producing IgG in blood from mice (n = 5) immunized with SNAP-AD 5 or untreated mice. Blood cells after lysis were stimulated with medium alone, 10 μg/mL of Igκ/Igλ antibodies, or 10 μg/mL 5-plex mixed antigen. (D) Quantification of B-cell producing IgG spots from (C) were analyzed by a one-way ANOVA with multiple comparison followed by Tukey's test, ∗ P<0.05, ∗∗∗∗P<0.0001 .

    Article Snippet: Mouse IFN-γ Single-Color ELISpot kit (ImmunoSpot, Cat# mIFNgp-2M/10) and Mouse IgG Single-Color ELISpot kit (ImmunoSpot, Cat# mIgG-SCE-2M/10) were purchased for ELISpot assays.

    Techniques: Enzyme-linked Immunospot, Control, Comparison, Lysis